Plate-based immunoassays are the workhorse of protein quantification, but these decades-old methods require tedious manual intervention, have limited dynamic range, require relatively large (50-100 μL) sample volume, and have long assay times.
The PIXI system consists of an assay cartridge and a corresponding module that sits on the deck of an automated liquid handler. Each assay kit includes the necessary reagents and a single 96-test cartridge. PIXI cartridges contain a proprietary nanoporous polymer, which accelerates the molecular interactions required to capture protein targets. After 30 min, users transfer the PIXI cartridge to your own imager to complete the readout. With a 6-log dynamic range and only 1 ul of sample volume required, this automated platform is a game-changer for your laboratory workflow.
PRODUCT WORKFLOW
The Definition of Plug and Play
Acheive data in as little as 30min with less than 2 min of hands on time
Load cartridge into PIXI and press GO to run automated ELISA
Transfer cartridge onto plate imager and read fluorescence output
Analyze ELISA results using integrated PIXI software
SPECIFICATIONS
General
Detection method
Fluorescence (640nm – 670nm)
Throughput
32, 64 or 96 samples at a time
Multiplex capability
Single target
Turnaround time (from sample loading to analyzed data)
75 min for 96 samples (max. throughput) 30 min for 32 samples (min. throughput)
Technical
Size
Ondeck Module
WDH: 12.8 cm x 33 cm x 7.3 cm, (5 in x 13 in x 3 in)
Waste Module
WDH: 16.5 cm x 38 cm x 20.5 cm, (6.5 in x 15 in x 8 in)
Weight
Ondeck Module
2.8 kg (6.25 lb)
Waste Module
7.5 kg (16.5 lb)
Electrical
100 – 240 VAC, 50/60 Hz, 300 VA
Compatible liquid handlers
The PIXI is compatible with most liquid handling robots working with 96 or 384 well plates. Ask Correlia about integrating with your specific liquid handler.
Imager compatibility
PIXI cartridges are currently compatible with many fluorescent cell imaging systems including the Biotek/Agilent Lionheart and Cytation line. Ask Correlia about your specific imager for more details.
Performance
Repeatability
10% CV from run-to-run
Precision
10% CV within a run
Dynamic log range
Up to 5 orders of magnitude
Sensitivity
~10 pg/mL for cytokines, 10 ng/mL for IgGs
ASSAYS
Off-the-Shelf
Pre-tested and ready-to-use without manipulation. Quality reagents, reproducible and robust results.
Custom
Develop your own assay. PIXI cartridges can be loaded with your unique targets of interests.