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Cell-free assembly and amplification aimed at replacing cell-based cloning
OriCiro offers ground breaking, cell-free technologies of large DNA assembly and amplification that can effectively replace the conventional E. coli cloning process.

OriCiro Research Service


Cell-free assembly and amplification aimed at replacing cell-based cloning

OriCiro offers ground breaking, cell-free technologies of large DNA assembly and amplification that can effectively replace the conventional E. coli cloning process.

Features
• Up to 50 DNA fragments can be assembled
• Maximum 200kb DNA fragment can be assembled as a circular DNA having oriC.
• Maximum 200kb circular DNA can be amplified
• 10μg and more circular DNA can be delivered
• 10,000 times more accurate amplification than PCR (Taq polymerase)


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Applications

  • Amplification of long DNA (up to 100kb) and sequences difficult to amplify by PCR
  • Efficient cloning of any DNA sequence including cytotoxic and repeat GC-rich
  • Making a library of Plasmid DNA for transfection
  • Phage/Virus genome production
  • DNA repair templates for gene editing in T cells therapy
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